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The substrate solution was then saved at −20°C right until the ammonium and nitrate contents were analyzed with the San++ Steady-Move Analysator (Skalar Analytic GmbH, Erkelenz, Germany). Nitrate determination was based on a response with hydrazinium sulfate, sulfanilamide and N-(1-naphthyl) ethylenediamine dihydrochloride which ends up in the f
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